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常用荧光染料的激发和发射波长
2020.11.30   点击3202次

常用荧光染料的激发和发射波长

这是一个用于流式细胞术或荧光显微镜的荧光色素的一些特性的表。Yarebio在组内,大致按激发波长的顺序排列(家族除外)。峰值激发和发射波长通常随探测器所处的环境而变化。所以需要自行确认所选染料的激发光谱和发射光谱。另外,下表显示的颜色只是一个非常粗略的近似值,并且不同显示设备会有一定色差!

ProbeEx (nm)Em (nm)MWNotes
Reactive and conjugated probes
Hydroxycoumarin325386331Succinimidyl ester
Aminocoumarin350445330Succinimidyl ester
Methoxycoumarin360410317Succinimidyl ester
Cascade Blue(375);401423596Hydrazide
Pacific Blue403455406Maleimide
Pacific Orange403551

Lucifer yellow425528

NBD466539294NBD-X
R-Phycoerythrin (PE)480;565578240 k
PE-Cy5 conjugates480;565;650670
aka Cychrome, R670, Tri-Color, Quantum Red
PE-Cy7 conjugates480;565;743767

Red 613480;565613
PE-Texas Red
PerCP490675
Peridinin chlorphyll protein
TruRed490,675695
PerCP-Cy5.5 conjugate
FluorX494520587(GE Healthcare)
Fluorescein495519389FITC; pH sensitive
BODIPY-FL503512

TRITC547572444TRITC
X-Rhodamine570576548XRITC
Lissamine Rhodamine B570590

Texas Red589615625Sulfonyl chloride
Allophycocyanin (APC)650660104 k
APC-Cy7 conjugates650;755767
PharRed
Alexa Fluor dyes [antibody conjugates] (Molecular Probes)
Alexa Fluor 350343442410
Alexa Fluor 4054014211028
Alexa Fluor 430434540702
Alexa Fluor 488499519643QY 0.92
Alexa Fluor 500503525700
Alexa Fluor 514517542714
Alexa Fluor 532530555724QY 0.61
Alexa Fluor 5465615721079QY 0.79
Alexa Fluor 5555535681250QY 0.1
Alexa Fluor 568579603792QY 0.69
Alexa Fluor 594591618820QY 0.66
Alexa Fluor 6106106291285
Alexa Fluor 6336326481200
Alexa Fluor 6476526681300QY 0.33
Alexa Fluor 6606636911100
Alexa Fluor 6806807021150
Alexa Fluor 7006967191400
Alexa Fluor 7507527761300
Alexa Fluor 7907828041750
Cy Dyes
Cy2489506714QY 0.12
Cy3(512);550570;(615)767QY 0.15
Cy3B558572;(620)658QY 0.67
Cy3.5581594;(640)1102QY 0.15
Cy5(625);650670792QY 0.28
Cy5.56756941128QY 0.23
Cy7743767818QY 0.28
DyLight dyes (Pierce)
DyLight 350353432

DyLight 405400420

DyLight 488493518

DyLight 549562576

DyLight 594593618

DyLight 633638658

DyLight 649654673

DyLight 680692712

DyLight 750752778

DyLight 800777794

Nucleic acid probes
Hoechst 33342343483616AT-selective
DAPI345455
AT-selective
Hoechst 33258345478624AT-selective
SYTOX Blue431480~400DNA
Chromomycin A3445575
CG-selective
Mithramycin445575

YOYO-14915091271
Ethidium Bromide493620394
Acridine Orange503530/640
DNA/RNA
SYTOX Green504523~600DNA
TOTO-1, TO-PRO-1509533
Vital stain, TOTO: Cyanine Dimer
TO-PRO: Cyanine Monomer
Thiazole Orange510530

Propidium Iodide (PI)536617668.4
LDS 751543;590712;607472DNA (543ex/712em), RNA (590ex/607em)
7-AAD546647
7-aminoactinomycin D, CG-selective
SYTOX Orange547570~500DNA
TOTO-3, TO-PRO-3642661

DRAQ5647681,697413(Biostatus) (usable excitation down to 488)
Cell function probes
Indo-1361/330490/4051010AM ester. Low/High Ca++,
Fluo-3506526855AM ester. pH > 6
DCFH5055355292'7'Dichorodihydrofluorescein, oxidized form
DHR505534346Dihydrorhodamine 123, oxidized form,
light catalyzes oxidation
SNARF548/579587/635
pH 6/9
Fluorescent Proteins

QYBRPSSource
Y66H360442





Y66F360508





EBFP380440
0.189
Addgenemonomer
EBFP2383448
0.5618
Addgenemonomer
Azurite383447
0.5514

monomer
GFPuv385508





T-Sapphire399511
0.602625
weak dimer
TagBFP40245726k0.6333++Evrogenmonomer
Cerulean433475
0.622736
weak dimer
mCFP433475
0.401364
monomer
ECFP434477
0.153


CyPet435477
0.511859
weak dimer
Y66W436485





dKeima-Red440616
0.318
MBLdimer
mKeima-Red440620
0.243
MBLmonomer
TagCFP458480
0.5729
Evrogendimer
AmCyan1458489
0.7529
Clontechtetramer
mTFP1 (Teal)462492
0.8554

dimer
S65A471504





Midoriishi-Cyan472495
0.925
MBLdimer
Wild Type GFP396,47550826k0.7716


S65C479507





TurboGFP48250226 k0.5337
Evrogendimer
TagGFP48250527k0.5934++Evrogenmonomer
TagGFP248350627k0.634++Evrogenmonomer
AcGFP148451027k0.8227
Clontech
S65L484510





Emerald487509
0.68390.69Invitrogenweak dimer
S65T488511





EGFP48850726k0.6034174Addgeneweak dimer
Azami-Green492505
0.7441
MBLtetramer (monomeric available)
ZsGreen1493505105k0.9140
Clontechtetramer
Dronpa-Green503518
0.8581
MBLphotoswitchable
TagYFP50852427k0.6247
Evrogenmonomer
EYFP51452726k0.615160
weak dimer
Topaz514527
0.6057

monomer
Venus515528
0.575315
weak dimer
mCitrine516529
0.765949
monomer
YPet517530
0.778049
weak dimer
TurboYFP52553826 k0.5356
Evrogendimer
PhiYFP52553726.8 k0.4052++Evrogenweak dimer
PhiYFP-m52553726.8 k0.3948++Evrogenmonomer
ZsYellow1529539
0.6513
Clontechtetramer
mBanana540553
0.704
Clontechmonomer
Kusabira-Orange548559
0.6031
MBLmonomer
mOrange548562
0.69499
monomer
mOrange2549565
0.6035
Clontechmonomer
mKO548559
0.6031122
monomer
TurboRFP55357426 k0.6762
Evrogendimer
tdTomato554581
0.699598Clontechtandem dimer
DsRed-Express2554591
0.4215
Clontech
TagRFP55558427k0.4848
Evrogenmonomer
DsRed monomer557592~28k0.13.516Clontechmonomer
DsRed2 ("RFP")563582~110k0.5524
Clontech
mStrawberry574596
0.292615Clontechmonomer
TurboFP60257460226 k0.3526
Evrogendimer
AsRed2576592~110k0.2113
Clontechtetramer
mRFP1584607~30k0.25

Tsien labmonomer
J-Red584610
0.208.813
dimer
mCherry587610
0.221696Clontechmonomer
HcRed1588618~52k0.030.6
Clontechdimer
mKate258863326k0.4025+Evrogenmonomer
Katushka (TurboFP635)58863526k0.3422++Evrogendimer
mKate (TagFP635)588635
0.3015
Evrogenmonomer
TurboFP63558863526 k0.3422
Evrogendimer
mPlum590649
0.104.153Clontech
mRaspberry598625
0.1513
Clontechmonomer; faster photobleach than mPlum
mNeptune600650
0.2013
Tsien Labmonomer
E2-Crimson611646
0.2329
Clontech
Other probes
Monochlorobimane380461226Glutathione probe
Calcein496517623pH > 5
HyPer420/500516
Hydrogen peroxide sensor

Legend:

  • Ex: Peak excitation wavelength (nm)

  • Em: Peak emission wavelength (nm)

  • MW: Molecular weight

  • QY: Quantum yield

  • BR: Brightness; Extinction coefficient * Quantum yield / 1000

  • PS: Photostability; time to 50% brightness (sec)


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